Test

Rickettsia PCR Urine

$230.00

The polymerase chain reaction (PCR)-based diagnostic assay for detection of Rickettsia from urine is highly specific to R. rickettsii and R. felis/typhi DNAs. The combination of the following three steps imparts a very high specificity and sensitivity to the test:

  1. Hybridization/selection
  2. Amplification of Rickettsia-specific DNA
  3. Detection of Rickettsia-specific amplified DNA fragments


Principle

The Rickettsia species PCR test is an assay that detects Rickettsia species-specific DNA in clinical samples. Rickettsia 17 kDa antigen gene fragments are hybrid-selected by probes, followed by PCR amplification of the selected DNA fragment. PCR products are tested with Rickettsia-specific probes (R. rickettsii and R. felis/typhi) by southern blot assays. The primers and probes used for the selection of a Rickettsia gene fragment encoding the 17 kDa antigen DNA fragments are designed from published sequences.

Tube / Specimen Requirements
Urine – BD Gray Top/ 4mL x2

CPT Code(s)
87798

Sample Report
View

Test Number 970 Categories , Tags , Test Methodology

PCR

Stage of Disease

Any Stage of Disease

Available in New York

No

• Enables accurate identification of biochemically unusual strains of pathogen.
• Facilitates much earlier detection of the microorganism because the assay is independent of the host’s immune response schedule.
Therefore, PCRs are useful for seronegative patients as well.
• Allows monitoring of the efficacy of an antibiotic regime.
• PCRs detect the DNA of the bacteria itself. Therefore, a positive result is definitive.

Positive: Rickettsia specific DNA detected.

Negative: Rickettsia specific DNA not detected

  • This test should only be performed in conjunction with southern blot assays.
  • Results should be interpreted in conjunction with other laboratory and clinical findings.
  • Test results can only help the physician in confirming a clinical diagnosis.

GET STARTED TODAY!

The first step in getting tested with IGeneX is to order a collection kit. Choose between a Blood, Urine, or Miscellaneous kit.

WHY IGENEX FOR TICK-BORNE DISEASE TESTING?

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