• Enables accurate identification of biochemically unusual strains of pathogen.
• Facilitates much earlier detection of the microorganism because the assay is independent of the host’s immune response schedule.
Therefore, PCRs are useful for seronegative patients as well.
• Allows monitoring of the efficacy of an antibiotic regime.
• PCRs detect the DNA of the bacteria itself. Therefore, a positive result is definitive.
Test
Rickettsia PCR – CSF
$230.00
The polymerase chain reaction (PCR)-based diagnostic assay for detection of Rickettsia from CSF is highly specific to R. rickettsii and R. felis/typhi DNAs. The combination of the following three steps imparts a very high specificity and sensitivity to the test:
- Hybridization/selection
- Amplification of Rickettsia-specific DNA
- Detection of Rickettsia-specific amplified DNA fragments
Principle
The Rickettsia species PCR test is an assay that detects Rickettsia species-specific DNA in clinical samples. Rickettsia 17 kDa antigen gene fragments are hybrid-selected by probes, followed by PCR amplification of the selected DNA fragment. PCR products are tested with Rickettsia-specific probes (R. rickettsii and R. felis/typhi) by southern blot assays. The primers and probes used for the selection of a Rickettsia gene fragment encoding the 17 kDa antigen DNA fragments are designed from published sequences.
Tube / Specimen Requirements
2mL CSF
CPT Code(s)
87798 x2
Positive: Rickettsia specific DNA detected.
Negative: Rickettsia specific DNA not detected
- This test should only be performed in conjunction with southern blot assays.
- Results should be interpreted in conjunction with other laboratory and clinical findings.
- Test results can only help the physician in confirming a clinical diagnosis.
- Only R. rickettsii will be reported for NY residents in Rickettsia PCR Panel
Related Tests
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The first step in getting tested with IGeneX is to order a collection kit. Choose between a Blood, Urine, or Miscellaneous kit.
WHY IGENEX FOR TICK-BORNE DISEASE TESTING?
- CLIA certified in all 50 states
- Long track record of success since 1991
- Offers testing that is nearly twice as accurate as CDC recommended tests
- Tests for all major tick-borne illnesses, including Lyme disease, TBRF, Babesia, Bartonella, and Rickettsia
- Utilizes comprehensive testing methods such as PCR, ImmunoBlots, IgXSpot and FISH
- Has become a nationwide leader in COVID-19 testing due to a relentless focus on customer satisfaction
