• Detects specific T-cell responses soon after infection, when antibodies are not detectable, or late in the disease when the levels of antibodies are low.
• When combined with Lyme ImmunoBlot tests, provides information on the full spectrum of a patient’s immune response to infection and stage of disease.
• Enables doctors to monitor disease progression and treatment.
• Especially useful for seronegative patients. They can be diagnosed with a Borrelia infection without antibody (IgM/IgG) production.
Test
Lyme IgXSpot
$295.00
The IgXSpot is an enzyme-linked immunospot assay that detects human T cells reactive to Borrelia/pathogen-specific antigens in vitro. It is well documented that both humoral and cellular immune responses develop in Borrelia/pathogen infection. The cellular immune response develops much earlier than humoral response in most patients who are infected with Borrelia species. In some patients, sero-conversion from cellular to humoral response does not occur or occurs much later in the disease. In some patients with a chronic form of the disease, the humoral response is poor. Therefore, the IgXSpot test is recommended for detection of very early and/or late Borrelia/pathogen infection and in seronegative patient’s whole blood samples.
Principle
ELISPOT is a widely used method for detecting and monitoring cellular immune responses to specific antigens. The IgXSpot assay allows visualization of the secretory product(s) of individual activated or responding cells to Borrelia-specific antigens. Each spot that develops in the assay represents a single reactive cell.
Tube / Specimen Requirements
1 Full Heparin (Must be received within 48 hours of collection at RT.)
CPT Code(s)
86352
Sample Report
View
Positive: > or = 3 SFU
Negative: < or = 2 SFU
Sample Viability cut-off: > 70%
SFU: Spot Forming Unit. Represents a reactive cell to B. burgdorferi Antigen(s).
For diagnostic purposes, the IgXSpot test results should be used in conjunction with clinical symptoms and other evidence available to the diagnosing physician. If the test result is negative, testing with another method is recommended. Depending on the stage of the disease patients can have either humoral or cellular immune response to infection. Thus, it is advisable to perform Lyme ImmunoBlot IgM and IgG tests with IgXSpot test.
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WHY IGENEX FOR TICK-BORNE DISEASE TESTING?
- CLIA certified in all 50 states
- Long track record of success since 1991
- Offers testing that is nearly twice as accurate as CDC recommended tests
- Tests for all major tick-borne illnesses, including Lyme disease, TBRF, Babesia, Bartonella, and Rickettsia
- Utilizes comprehensive testing methods such as PCR, ImmunoBlots, IgXSpot and FISH
- Has become a nationwide leader in COVID-19 testing due to a relentless focus on customer satisfaction
