Test

Lyme Dot Blot (3 samples)

$255.00

Lyme Dot-Blot Assay (LDA) is a qualitative immunoassay for the direct detection of Borrelia burgdorferi-specific antigens in a patient urine sample using anti-B. burgdorferi antibodies.

Purpose
The antigens present in the urine sample are immobilized onto a membrane in a Dot-blot format and incubated with anti-B. burgdorferi-specific antibodies. After washing the bound anti-B. burgdorferi-specific antibodies, they are reacted with anti-rabbit IgG, which is visualized by an enzyme/substrate reaction.

Tube / Specimen Requirements
Urine – BD Gray Top/ 4mL x6 (2 tubes per collection day)

CPT Code(s)
87449 x3

Test Number 805 Categories , Tags , Stage of Disease

Any Stage of Disease

Test Methodology

LDA

Available in New York

No

• Requires urine samples, and are therefore more practical tests for babies and infants, as the sample is easier to catch.
• Doctors are able to run different antibiotic challenges to determine
whether treatment is working to draw out the Lyme bacteria in the urine.
• If the initial Lyme panel tests on a patient’s blood samples are negative, including PCR, but symptoms for Lyme disease are present, the Lyme Dot-blot assay on urine can be helpful in making the diagnosis.

Positive: B. burgdorferi antigens
detected

Negative: B. burgdorferi antigens not
detected

  1. Negative tests do not exclude diagnosis of Lyme disease
  2. Cross-reactions with Leptospira may occur.
  3. False positive may occur for patients with a Urinary Tract Infection (UTI), currently being treated for a UTI, or who have undergone treatment for a UTI within the last two weeks.
  4. Results should be interpreted in conjunction with other laboratory and clinical findings.

GET STARTED TODAY!

The first step in getting tested with IGeneX is to order a collection kit. Choose between a Blood, Urine, or Miscellaneous kit.

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