Test

B. henselae PCR – CSF

$265.00

The Bartonella henselae Polymerase Chain Reaction (PCR) test is an assay that detects B. henselae-specific DNA in cerebral spinal fluid (CSF). The combination of the following three steps imparts a very high specificity and sensitivity to the test:

1. Hybridization/selection
2. Amplification of Bartonella-specific DNA
3. Detection of Bartonella-specific amplified DNA fragments

Principle
B. henselae ribosomal DNA (rDNA) fragments are hybrid-selected by probes, followed by PCR amplification of selected B. henselae rDNA. PCR products are confirmed by B. henselae-specific probes in a southern blot assay. The primers and probes used for the selection of B. henselae rDNA fragments are designed from published small subunit ribosomal RNA sequences.

Tube / Specimen Requirements
2mL CSF

CPT Code(s)
87471

Test Number 281 Categories , Tags , Stage of Disease

Any Stage of Disease

Test Methodology

PCR

Available in New York

Yes

• Enables accurate identification of biochemically unusual strains of pathogen.
• Facilitates much earlier detection of the microorganism because the assay is independent of the host’s immune response schedule.
Therefore, PCRs are useful for seronegative patients as well.
• Allows monitoring of the efficacy of an antibiotic regime.
• PCRs are detecting the DNA of the bacteria itself. Therefore, a positive result is definitive.

Positive: B. henselae rDNA detected.
Negative: B. henselae rDNA not detected

  1. Bartonella PCR results should be interpreted in conjunction with other laboratory and clinical findings.
  2. Test results can only help the physician in confirming a clinical diagnosis.

GET STARTED TODAY!

The first step in getting tested with IGeneX is to order a collection kit. Choose between a Blood, Urine, or Miscellaneous kit.

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