• Enables accurate identification of biochemically unusual strains of pathogen.
• Facilitates much earlier detection of the microorganism because the assay is independent of the host’s immune response schedule. Therefore, PCRs are useful for seronegative patients as well.
• The assay can be performed on any type of sample: whole blood, serum, urine, tissue, breast milk, cerebral spinal fluid, synovial fluid, and ticks.
• PCRs are detecting the DNA of the bacteria itself. Therefore, a positive result is definitive.
• The test also detects DNA to other Lyme Borreliae: B. afzeli, B. andersonii, B. garinii and B. mayonii.
Test
Lyme Multiplex PCR Urine (pooled)
$265.00
The Lyme Multiplex PCR test detects Borrelia burgdorferi specific DNA sequences from Osp A plasmid and flagellin genomic genes, in clinical specimens.
Principle
The multiplex PCR-based diagnostic test is performed directly on the clinical specimen in a three-step assay. The steps are as follows:
Selection hybridization
Specifically removes the “common PCR inhibitors” from the clinical sample while simultaneously selecting and purifying the DNA fragment of interest. This procedure also concentrates the fragment of interest, thereby improving sensitivity.
PCR amplification
The purified pathogen DNA fragment of interest is PCR-amplified with pathogen-specific primers. This sequence “hybridizes” or binds specifically to pathogen DNA of interest under predetermined PCR conditions. Therefore, only pathogen-specific DNA is amplified.
Detection of amplified products
The PCR-amplified products are transferred and bound to a nitrocellulose membrane. The membrane-bound, PCR-amplified products are hybridized with pathogen-specific probes. Only samples that have pathogen-specific DNA hybridize to the probes and give a blue-purple color dot on the membrane.
The combination of these three steps imparts very high specificity and sensitivity to the test.
Tube / Specimen Requirements
Urine – BD Gray Top/ 4mL x6 (2 tubes per collection day)
CPT Code(s)
87801 x2
Genomic
Positive: B. burgdorferi DNA detected.
Negative: B. burgdorferi DNA not detected.
Plasmid
Positive: B. burgdorferi DNA detected.
Negative: B. burgdorferi DNA not detected.
Sample is considered positive if either genomic or plasmid is positive.
- Results should be interpreted in conjunction with other laboratory and clinical findings.
- Test results can only help the physician in confirming clinical diagnosis.
Related Tests
GET STARTED TODAY!
The first step in getting tested with IGeneX is to order a collection kit. Choose between a Blood, Urine, or Miscellaneous kit.
WHY IGENEX FOR TICK-BORNE DISEASE TESTING?
- CLIA certified in all 50 states
- Long track record of success since 1991
- Offers testing that is nearly twice as accurate as CDC recommended tests
- Tests for all major tick-borne illnesses, including Lyme disease, TBRF, Babesia, Bartonella, and Rickettsia
- Utilizes comprehensive testing methods such as PCR, ImmunoBlots, IgXSpot and FISH
- Has become a nationwide leader in COVID-19 testing due to a relentless focus on customer satisfaction
